New Cell Therapy Aids Heart Recovery—Without Implanting Cells

Columbia biomedical engineers invent innovative technique to help injured hearts regenerate, through therapeutic application of extracellular vesicles secreted by cardiomyocytes derived from human pluripotent stem cells

Apr 23 2018 | By Holly Evarts | Image: Gordana Vunjak-Novakovic/Columbia Engineering

New York, NY—April 23, 2018—Heart disease is a major global health problem—myocardial infarction annually affects more than one million people in the U.S. alone, and there is still no effective treatment. The adult human heart cannot regenerate itself after injury, and the death of cardiac muscle cells, known as cardiomyocytes, irreversibly weakens the heart and limits its ability to pump blood.

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About the Study

The study is titled “Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells.”
Authors, all from Columbia University, are: Bohao Liu (Department of Medicine and Department of Biomedical Engineering); Benjamin W. Lee (Department of Medicine and Department of Biomedical Engineering, currently resident at the University of Pennsylvania); Koki Nakanishi (Department of Medicine); Aranzazu Villasante (Department of Biomedical Engineering); Rebecca Williamson (Department of Pathology and Cell Biology); Jordan Metz (Department of Systems Biology); Jinho Kim (Department of Biomedical Engineering); Mariko Kanai (Department of Medicine); Lynn Bi (Department of Biomedical Engineering); Kristy Brown (Department of Pathology and Cell Biology); Gilbert Di Paolo (Department of Pathology and Cell Biology); Shunichi Homma (Department of Medicine); Peter A. Sims (Department of Systems Biology and Department of Biochemistry and Molecular Biophysics); Veli K. Topkara (Department of Medicine); and Gordana Vunjak-Novakovic (Department of Biomedical Engineering and Department of Medicine).
The study was funded by the National Institutes of Health: National Heart, Lung and Blood Institute (HL076485), National Institute for Biomedical Imaging and Bioengineering (EB002520, EB17103) National Institute for General Medical Sciences (GM007367), the National Institute of Aging (F30 AG047748), NYSTEM (C028119), and the Lisa and Mark Schwartz Program for Reversing Heart Failure.
The authors declare no financial or other conflicts of interest.

Recovery of the injured heart muscle after 4 weeks of treatment with extracellular vesicles. Immunostains: wheat germ agglutinin (red), troponin (green) and DAPI (blue).

Researchers have turned their focus to stem cell transplantation for cardiomyocyte replacement and recovery of heart function, but studies have shown that implanted stem cells have difficulty surviving and differentiating into cardiomyocytes to repair the damaged muscle. When stem cells were differentiated into cardiomyocytes before implantation, heart function improved, but with a complication: the implanted cardiomyocytes did not contract synchronously with the heart, thus causing potentially lethal arrhythmias (abnormal heart rhythm).

A team of Columbia University investigators, led by Biomedical Engineering Professor Gordana Vunjak-Novakovic, has designed a creative new approach to help injured hearts regenerate by applying extracellular vesicles secreted by cardiomyocytes rather than implanting the cells. The study, published online today in Nature Biomedical Engineering, shows that the cardiomyocytes derived from human pluripotent stem cells (derived in turn from a small sample of blood) could be a powerful, untapped source of therapeutic microvesicles that could lead to safe and effective treatments of damaged hearts.

Cell-secreted microvesicles are easy to isolate and can be frozen and stored over long periods of time. Such an “off-the-shelf” product has several major advantages over cell therapy—1) it can be used immediately in an acute-care setting, unlike cells that can take months to isolate and grow; 2) it does not cause arrhythmia (which often occurs when cells are transplanted); and 3) the regulatory path towards clinical application is much simpler than for a cell-based therapy. 

It is well known from numerous clinical studies that most of the implanted stem cells are washed away within hours of the treatment, but there still are beneficial effects. This has led to the informal “hit-and-run” hypothesis, meaning that the cells deliver their cargo of regulatory molecules before leaving the site of injury. “Consistent with this hypothesis, we postulated that the benefits of cell therapy of the heart could be coming from the secreted bioactive molecules (such as micro RNAs), rather than the cells themselves,” says Vunjak-Novakovic, the study’s senior author, University Professor, The Mikati Foundation Professor at Columbia Engineering, and professor of medicine at Columbia University Vagelos College of Physicians and Surgeons. “So we explored whether the benefits of cell therapy of the injured heart could be achieved without using the cells. This way, we would largely simplify the translation into the clinic, and avoid the burden of arrhythmia associated with implantation of contractile cells.”

Nearly all cells secrete and uptake tiny extracellular vesicles that are filled with genetic messages that can influence recipient cells. These extracellular vesicles are like letters that cells use naturally to communicate with their neighbors, both near and far, within the body.

“We reasoned that the cardiomyocytes would be the best source of molecules driving the recovery of injured heart, as it is well known that these cells can build muscle when used in tissue-engineering models,” says Bohao Liu, the paper’s co-lead author and MD/PhD candidate in Columbia Engineering’s department of biomedical engineering. “I’m very excited about our promising results, and I believe that the cell-free therapy represents a step in the right direction for developing safe and effective treatments of the infarcted heart.”

The interdisciplinary team, which included bioengineers, clinicians, and systems biology scientists, derived cardiomyocytes from adult human stem cells and cultured these cells to allow them to secrete extracellular vesicles. The vesicles secreted by undiffereniated stem cells were used for comparison. The researchers then used next-generation sequencing to read their messages and instructions. They found that the extracellular vesicles from cardiomyocytes—but not from stem cells—contained cardiogenic and vasculogenic microRNAs that are very powerful regulatory molecules.

Building on the expertise of Vunjak-Novakovic’s lab in biomaterials and hydrogels, the team encapsulated the vesicles in a collagen-based patch that slowly released them over the course of four weeks when implanted onto the injured heart in rat models of myocardial infarction. The researchers monitored the heart to measure blood-pumping function and look for any signs of arrhythmia.

“We were really excited to find that not only did the hearts treated with cardiomyocyte extracellular vesicles experienced much fewer arrhythmias, but they also recovered cardiac function most effectively and most completely,” says Vunjak-Novakovic. “In fact, by four weeks after treatment, the hearts treated with extracellular vesicles had similar cardiac function as those that were never injured.”

The team plans next to determine how much the properties of extracellular vesicles change with the maturation of cardiomyocytes derived from pluripotent stem cells, and what would be the optimal developmental stage, or level of maturity, of cardiomyocytes to maximize therapeutic outcomes. They are also exploring the specific mechanisms behind the therapeutic effects observed in this study.

“Once we better understand how exactly the extracellular vesicles do what they do,” Vunjak-Novakovic adds, “we should be able to extend their use to a range of cardiovascular diseases, and significantly advance the field of cell-free heart therapy.”


Columbia Engineering
Columbia Engineering, based in New York City, is one of the top engineering schools in the U.S. and one of the oldest in the nation. Also known as The Fu Foundation School of Engineering and Applied Science, the School expands knowledge and advances technology through the pioneering research of its more than 200 faculty, while educating undergraduate and graduate students in a collaborative environment to become leaders informed by a firm foundation in engineering. The School’s faculty are at the center of the University’s cross-disciplinary research, contributing to the Data Science Institute, Earth Institute, Zuckerman Mind Brain Behavior Institute, Precision Medicine Initiative, and the Columbia Nano Initiative. Guided by its strategic vision, “Columbia Engineering for Humanity,” the School aims to translate ideas into innovations that foster a sustainable, healthy, secure, connected, and creative humanity.

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